Botryosphaeriales » Botryosphaeriaceae » Neofusicoccum

Neofusicoccum parvum

Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips, Stud. Mycol. 55: 248 (2006)

Index Fungorum number: IF500879         Facesoffungi number: FoF02411

 

Sexual morph: Ascostromata 155–250 μm diameter, 115–190 μm high (including the papilla), black dots and convex on host tissue, appearing through cracks in bark, solitary or clustered, when cut horizontally locules visible as white contents and dark ascospore dots, semiimmersed to erumpent under epidermis, individually globose to subglobose, with papilla. Ostiole central, circular, papillate. Peridium 25–65 μm, comprising two layers, outer layer composed of dark brown to black, thick-walled cells of textura angularis, inner layer composed of hyaline, thinwalled cells of textura angularis. Hamathecium comprising 2–4 μm wide, hyaline, hyphae-like, numerous, septate, pseudoparaphyses, slightly constricted at the septa, embedded in gelatinous matrix. Asci 55−135 × 16−23 μm (x̄ = 84 × 19 μm, n = 20), 8-spored, bitunicate, fissitunicate, clavate to cylindro-clavate, short to long pedicellate, with ocular chamber, apically rounded. Ascospores 17−25.5 × 7–10 μm (x̄ = 20 × 8 μm, n = 20), uniseriate at the base, 2–3-seriate at the centre and end, hyaline, ellipsoidal to fusiform, aseptate, wider in the centre, rounded or acute at the ends, thick-walled. Asexual morph: Undetermined.

 

Culture characteristics: Colonies on PDA fluffy, fairly dense, aerial, raised, filamentous, pigments not produced, white at first, becoming gray to grey-black after one to two weeks.

 

Habitat: Saprobic on dead branch.

 

Known hosts: Actinidia deliciosa, Heteropyxis natalensis, Malus sylvestris, Populus nigra, Eucalyptus sp., E. grandis, E. smithii, Ribes sp., Sequoia gigantea (Slippers et al. 2004), Acer pseudoplatanus, Quercus robur (Moricca et al. 2012), Actinidia deliciosa, A. chinensis, Populus nigra, Eucalyptus obliqua, Prunus cerasoides (Trakunyingcharoen et al. 2015), Pinus nigra, Vitis vinifera (Crous et al. 2006a, Phillips et al. 2008), Aesculus hippocastanum, Eucalyptus globulus, Ferula communis (Lopes et al. 2016), Juglans regia, Salix sp., Sclerocarya birrea subsp. caffra (Mehl et al. 2017), Avicennia marina, Bruguiera gymnorrhiza, Lumnitzera racemose, Rhizophora mucronata (Osorio et al. 2017), Linum usitatissimum (Liu et al. 2012b), Vitis vinifera (Alves et al. 2005), Mangifera indica (Mehl et al. 2017,), Hyde et al. 2020). For more information see Farr & Rossman (2020).

 

Known distribution: worldwide e.g. Algeria (Berraf-Tebbal et al. 2014), Australia, Indonesia (Sumatra), Brazil, China (Sichuan), France (Van Niekerk et al. 2004), Italy (Milan) (Moricca et al. 2012), New Zealand (TePuke) (Alves et al. 2005), Portugal (Palmela) (Phillips et al. 2008), (Hogsback, Kwazulu-Natal, Mpumalanga) (Slippers et al. 2004), (e.g. Gauteng, Limpopo) (Mehl et al. 2017), USA (California) (Úrbez-Torres et al. 2007), (Hawaii) (Slippers et al. 2004), Thailand (Chiang Mai Province) (Liu et al. 2012b, Trakunyingcharoen et al. 2015), (Phayao Province) (this study), For more information see Farr & Rossman (2020).

 

Material examined: Thailand, Phayao Province, Muang District, on dead branch of Mangifera indica (Anacardiaceae), 12 March 2012, M. Doilom (MFLU 19–1562, new host record), living culture (MFLUCC 12–0380).

 

GenBank Accession No: ITS: MN582744, TEF1: MN629744, TUB: MN643160

 

Notes: Neofusicoccum parvum has been previously reported on Eucalyptus obliqua, Linum usitatissimum and Prunus cerasoides in Thailand (Liu et al. 2012b, Trakunyingcharoen et al. 2015). The holotype is from dead branches of Populus nigra in New Zealand (Pennycook & Samuels 1985). Although this species is widely distributed on mango in many countries such as Australia, Brazil, Italy, México, Peru, Puerto Rico, Spain and Taiwan (Javier-Alva et al. 2009, Ni et al. 2012, Ismail 2013, Sandoval-Sanchez et al. 2013, Serrato-Diaz et al. 2013, Arjona-Girona & LopezHerrera 2016), it has not yet been reported on Mangifera indica (mango) in Thailand (Farr & Rossman 2020). Conidial size of our specimen is similar to the type N. parvum (20 × 8 μm vs 20.8 × 9.2 μm) (Pennycook & Samuels 1985) as well as the N. parvum isolate identified by Liu et al. (2012b) (20 × 8 μm). The isolate MFLUCC 12-0380 clustered with the ex-type strain of N. parvum (CMW 9081) and N. parvum (MFLUCC 11-0184) (Fig. 112). Thus we identify our collection as N. parvum and this is the first report of N. parvum on Mangifera indica in Thailand.