Botryosphaeriales » Botryosphaeriaceae » Lasiodiplodia

Lasiodiplodia pseudotheobromae

Lasiodiplodia pseudotheobromae A.J.L. Phillips, A. Alves & Crous

Facesoffungi number: FoF04567

 

Saprobic on dead leaves of Pandanus sp. Sexual morph: Undetermined. Asexual morph: Conidiomata 165–240 × 140–215 µm (x̄ = 193.6 × 180 µm, n = 5), scattered to gregarious, immersed, conspicuous on host surface, dark brown, solitary, uniloculate, obpyriform, ostiole in the central, without papilla, always sporulate on host surface. Pycnidial wall 10–35 µm, composed of several layers, thick-walled, pale brown to dark brown cells of textura angularis. Conidiogenous cell 4.5–8 × 2.5–8 μm (x̄ = 6 × 5 μm, n = 10), holoblastic, cylindrical, flat at the base, thick-walled, smooth. Conidia 20–30 × 10–15 µm (x̄ = 24 × 12.4 µm, n = 30), oval to ellipsoid, 1-septate with age, dark band at the septum, not constricted at septum, initially hyaline becoming yellow brown or brown with age, rounded at apex, guttulate, longitudinal striations from apex to base, without mucilaginous sheath.

 

Culture characteristics: Conidia germinating on MEA within 12 hr. Colonies on MEA, white at first and becoming black with age, circular, with entire edge, raised, velvety, black in reverse, with smooth margin.

 

Material examined: THAILAND, Chiang Rai Province, Muang District, Mae Fah Luang university, on dead leaf of Pandanus sp., 15 December 2017, S. Tibpromma P08 (MFLU 18-0027, HKAS 101796); living culture, MFLUCC 18-0114, GenBank numbers LSU: MH376724; ITS: MH388351; SSU: MH388318; TEF1: MH388386; TUB2: MH412719.

 

Notes: In the phylogeny our collection clusters between Lasiodiplodia pseudotheobromae (CBS 447.62) and L. pseudotheobromae (CBS 116459), first reported on grapevine in Brazil as a trunk pathogen (Correia et al. 2013). Conidial dimensions of our collection are similar to the values given by Alves et al. (2008). This is the first record of L. pseudotheobromae on dead leaves of Pandanus sp.

 

Figure X. Lasiodiplodia pseudotheobromae (MFLU 18-0027). a, b Appearance of conidiomata on host substrate. c Section of conidioma. d Section of pycnidial wall. e-g Conidiogenous cell producing conidia. h-j Conidia. k Germinating conidium. l, m Colony on MEA from above and below. Scale bars: c = 50 μm, d-k = 5 μm.

 

Reference: Tibpromma S, Hyde KD, McKenzie EHC, Bhat DJ, et al. (2018b) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Divers 92:1–160.

 

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