Botryosphaeriales » Botryosphaeriaceae » Lasiodiplodia

Lasiodiplodia pandanicola

Lasiodiplodia pandanicola Tibpromma & K.D. Hyde, in Fungal Diversity 92: 58 (2018)

Index Fungorum number: IF554473         Facesoffungi number: FoF04566


Sexual morph: Undetermined. Asexual morph: Conidiomata 185– 210 9 187–240 μm ( = 198 × 211 μm, n = 5), immersed to erumpent through host, visible as black, subglobose to ovoid, solitary, scattered or gregarious, ostiole. Pycnidial wall 20–42 μm, composed of several layers of textura angularis, dark brown. Conidiophores micronematous, reduced to conidiogenous cell. Conidiogenous cells 4–6.5 × 5–7 μm ( = 5 × 6 μm, n = 5), phialidic, integrated, cylindrical or cylindric-clavate or irregular swollen cell, hyaline, smooth. Conidia 14– 38 × 9–22 μm ( = 27 × 14 μm, n = 30), ellipsoidal to obovate, initially hyaline and aseptate, later becoming brown and 1-septate, thick and rough-walled, guttulate, with longitudinal striations.


Culture characteristics: Conidia germinating on MEA within 12 h. Colonies on MEA, initially white–grey and later become black, with dense mycelium, circular, with entire edge, raised, velvety, black in reverse, with smooth margin. Sporulating in culture after 2months, producing conidia similar in shape to those recorded on natural dead leaves.


Material examined: THAILAND, Phatthalung Province, Mueang Phatthalung District, on dead leaves of Pandanus sp., 14 June 2015, B. Thongbai SF15-009 (MFLU 18-0011, holotype); ex-type living culture, MFLUCC 16-0265 = KUMCC 16-0158.


GenBank Accession No: LSU: MH260301; ITS: MH275068; TEF1: MH412774; TUB2: MH412744.


Notes: Phylogenetic analyses indicated Lasiodiplodia pandanicola is well-separated with other Lasiodiplodia spp. (95% in ML, 1 in BYPP). Lasiodiplodia parva A.J.L. Phillips, A. Alves & Crous share similar conidia to L. pandanicola. However, L. parva has conidia 16–23.5 × 10.5–13 μm, ovoid (Alves et al. 2008), while L. pandanicola has conidia 14–38 × 9–22 μm and ellipsoidal to obovate. Therefore, we propose L. pandanicola as a new species. In a BLASTn search on NCBI GenBank, the closest matches of ITS sequence of MFLUCC 16-0265 is L. theobromae with 100% identity to the strain Lt-A2 (KX270362), while the closest matches with the TEF1 sequence were with 100% identical L. theobromae strain IRNHM-KB64-2 (KU737511) and closest matches with the TUB2 sequence were with 95% identical Botryosphaeria quercuum strain CBS177.89 (DQ026404).

Figure X. Lasiodiplodia pandanicola (MFLU 18-0011, holotype). a Appearance of conidiomata on host substrate. b Section of conidioma. c Section of pycnidial wall. d Conidiogenous cell producing conidia. e–g Conidia. h Conidium with longitudinal striations. i Germinating conidium. j, k Colony on MEA from above and below. l, m Conidia formed in culture. Scale bars: a = 200 μm, b = 100 μm, c, d, l, m = 20 μm, e–i = 5 μm.


Reference: Tibpromma S, Hyde KD, Bhat JD, Mortimer PE, Xu JC, Promputtha I, Doilom M, Yang JB, Tang AMC, Karunarathna SC (2018) Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand. MycoKeys. 33:25-67.


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"The future of specialist fungi in a changing climate: baseline data for generalist and specialist fungi associated with ants Rhododendron species and Dracaena species"

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