Lasiodiplodia hyalina
Lasiodiplodia hyalina Zh.P. Dou & Y. Zhang, Mycosphere 8 (2): 1016 (2017)
Facesoffungi number: FoF04565
Saprobic on dead leaves of Pandanus sp. Sexual morph: Undetermined. Asexual morph: Conidiomata 197–244 × 134–156 µm ( x̄ = 222.6 × 148.5 µm, n = 5), scattered to gregarious, immersed, conspicuous on host surface, dark brown, solitary, uniloculate, obpyriform, ostiole central, without papilla, always sporulating on host surface. Pycnidial wall 22–55 µm, composed of several layers of thick-walled, pale brown to dark brown cells of textura angularis. Conidiogenous cells 5–10 × 4–7 μm (x̄ = 7 × 5.5 μm, n = 10), holoblastic, phialidic, ovoid, thick-walled, smooth. Conidia 20–30 × 10–15 µm (x̄ = 23 × 12 µm, n = 30), oval, becoming 1-septate with age, with a dark band at septum, not constricted at septum, initially hyaline to yellow brown or brown with age, rounded at both ends, guttulate, with longitudinal surface striations from apex to base, without mucilaginous sheath.
Culture characteristics: Conidia germinating on MEA within 12 hr. Colonies on MEA, white at first, becoming black with age, circular, with entire edge, raised, velvety, black in reverse, with smooth margin.
Material examined: THAILAND, Chiang Mai Province, Mae Taeng District, Mushroom Research Foundation, on dead leaf of Pandanus sp., 16 December 2017, S. Tibpromma P04 (MFLU 18-0023 = HKAS 101792); living culture, MFLUCC 18-0110, GenBank numbers LSU: MH260300; ITS: MH275067; SSU: MH260341; TUB2: MH412743.
Notes: Our isolate similar with Lasiodiplodia hyalina Z.P. Dou & Ying Zhang which found from cankered stems of Acacia confuse and unidentified woody plant (Dou et al. 2017). Lasiodiplodia hyalina has cylindrical to ampulliform conidiogenous cells and ellipsoid to ovoid, 24 × 13.6 μm conidia (Dou et al. 2017) and similar to our isolate. Lasiodiplodia hyalina (MFLUCC 18-0110) is supported by our molecular data (Fig. 41) and this is the first record of L. hyalina on dead leaves of Pandanus sp.
Figure X. Lasiodiplodia hyalina (MFLU 18-0023). a Appearance of conidiomata on host substrate. b Section of conidioma. c Section through ostiole. d Pycnidial wall. e-g Conidiogenous cell producing conidia. h-j Conidia. k Conidium with dark longitudinal striations. l Germinating conidium. Scale bars: b = 50 μm, c = 20 μm, d = 10 μm, e-l = 5 μm.
Reference: Tibpromma S, Hyde KD, McKenzie EHC, Bhat DJ, et al. (2018b) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Divers 92:1–160.
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