Botryosphaeriales » Botryosphaeriaceae » Lasiodiplodia

Lasiodiplodia brasiliensis

Lasiodiplodia brasiliensis M.S.B. Netto, M.W. Marques & A.J.L. Phillips [as “brasiliense”], Fungal Diversity 67: 134 (2014)

Facesoffungi number: FoF 00628.

 

Description: Saprobic on dead branch of Tectona grandis. Sexual morph: Undetermined. Asexual morph: Conidiomata pycnidial formed on MEA after 1 month, dark brown, erumpent. Paraphyses up to 60 μm long, 1.5–5.5 μm wide, hyaline, septate, cylindrical, ends rounded, numerous. Conidiogenous cells 6–15 × 1.5–6 μm ( = 10 x 3.5 μm n = 20), holoblastic, hyaline, cylindrical. Conidia on host (22–)26–27 (–29) × 12–16 μm ( ± S.D. = 26 ± 1.6 x 14 ± 1 μm n = 30), in culture (19–)25–27(–28) × 12–17 μm ( ± S.D. = 25 ± 2 x 15 ± 1 μm n = 30), initially hyaline and aseptate, becoming 1-septate, dark brown, thick-walled, ellipsoid to obovoid, guttulate, apex broadly rounded, base truncate or round, with longitudinal striations from apex to base.

 

Culture characteristics: Conidia germinating on PDA after 5 h. Germ tubes produced from both ends of conidia. Colonies on MEA reaching 50 mm diameter after 1 day in the dark at 25 °C, fast growing, raised, fluffy, undulate, dense, filamentous, convex with papillate surface, initially white, after 2 days becoming pale grey and becoming dark grey (1F1) after 1 week, reaching the edge of the Petri-dish after 2 days.

 

Notes: Lasiodiplodia brasiliense collected in this study differs from the type species in having septate paraphyses, although this may have been overlooked in the type (Netto et al. 2014). They also differ in hosts. In this study L. brasiliense was collected from T. grandis, while the type was collected from Mangifera indica. Our collection from Tectona grandis is illustrated and described here to facilitate identification from this host.