Barriopsis tectonae

Barriopsis tectonae Doilom, L.A. Shuttlew. & K.D. Hyde, in Doilom, Shuttleworth, Roux, Chukeatirote & Hyde, Phytotaxa 176(1): 84 (2014)

Index Fungorum number: 808202

Saprobic on dead branch of Tectona grandis. Sexual morph: Ascostromata (195–) 280–325 (–365)   m high × (230–)265–285(–320)   m diam. ( x = 280 × 265   m, n = 10), black, initially immersed, becoming erumpent through bark fissures, solitary or gregarious, uniloculate, globose to subglobose, or flask-shaped, when cut horizontally, locules visible with white contents and dark ascospore dots, papillate. Papilla 100–115 μm long, 100–120 μm diam, ostiole with periphyses. Peridium composed of several layers of dark brown-walled cells of textura angularis. Hamathecium comprising 2.5–6 μm wide, hyphae-like, numerous, septate, pseudoparaphyses, slightly constricted at septa. Asci (120–)167–185(–200) ×(28–)30.5–32(–35) m (x̅ =160 × 31 m, n = 10), 8- spored, bitunicate, fissitunicate, cylindro-clavate or clavate, with long or short pedicel, apically rounded with an ocular chamber. Ascospores (26–)29–30 (–33) × (13–)14.5–15(–17) m (x̅ = 29 × 15 m, n = 30), overlapping biseriate, initially hyaline, becoming pale brown or reddish-brown when mature, aseptate, ellipsoid to ovoid, ends rounded, without terminal apiculi at each end, thick-walled, smooth or verruculose, swollen in the centre. Asexual morph: Conidiomata pycnidia, superficial on toothpick in agar media, covered with dense olivaceous to olivaceous- black mycelium, solitary, or gregarious, scattered, globose to sub globose, unilocular. Paraphyses not observed. Conidia (29–)37–37.5(–38) × 15.5–17.5(–18) m (x̅ = 36 × 17 m, n = 10), initially hyaline and aseptate, becoming pale brown to dark brown and 1–2 septate, thick-walled, smooth, ellipsoid to obovoid, apex broadly rounded, base rounded to truncate, with longitudinal striations when mature, striations not present when immature, granular contents. Chlamydospores catenate, intercalary and terminal, hyaline becoming brown to dark brown with maturity, thick-walled, smooth, granular contents.

Culture characteristics: Ascospores germinating on PDA after 5–12 h. Germ tubes produced from germ pore of ascospores. Only asexual morph produced in culture. Colonies on MEA reaching 35–40 mm diam after 4 days in the dark at 25°C, flattened, velvety, lobate, fimbriate, initially white, after 2 days becoming olive (3F5) in the centre, white at the edge, reaching the edge of the Petri dish after 15 days. Cardinal temperatures for growth after four days and colony diameter (mm): optimum 25°C, no growth at 5°C, 6 mm at 10°C, 16 mm at 15°C, 27 mm at 20°C, 37 mm at 25°C, 13 mm at 30°C, no growth at 35°C.

Material examined: THAILAND. Phayao Province: Muang District, on dead branch of Tectona grandis, 12 March 2012, M. Doilom (MFLU 14-0024, holotype, ex-type culture MFLUCC 12-0381 = CMW 40687 = CBS 137786).

Figure X. Barriopsis tectonae (MFLU 14-0024, holotype) A. Ascostromata on host. B. Ascostromata cut through horizontally showing the white contents with dark spots. C–D. Section through ascoma on host. E. Papilla with periphyses. F. Peridium. G. Pseudoparaphyses. H. Immature ascus. I–J. Immature ascus with hyaline ascospores, mature asci with brown ascospores. K, M. Mature ascospore/s without terminal apiculi. L. Immature ascopsore without longitudinal striations. N. Germinated ascospore. O. Immature conidium. P. Mature conidium with two septa. Q, R. Mature conidia with longitudinal striations. Scale bars: A, B = 500 µm, C = 100 µm, D, K = 50 µm, E, H–J, N = 20 µm, F, G, L, M, O–R = 10 µm.

References: Doilom M, Shuttleworth LA, Roux J, Chukeatirote E, et al. (2014) Barriopsis tectonae sp. nov. a new species of Botryosphaeriaceae from Tectona grandis (teak) in Thailand. Phytotaxa 176(1): 81-91.