Barriopsis stevensiana
Barriopsis stevensiana A.J.L. Phillips & Pennycook, Fungal Diversity, 86: 56 (2017)
Index Fungorum number: IF819573 Facesoffungi number: FoF07061
Sexual morph: Ascostromata individual locule 150–280 μm high × 215−300 μm diameter (ascostromata with papilla), black, convex on host tissue, appearing through cracks in bark, solitary, initially immersed, becoming erumpent, when cut 159 horizontally locules visible as white contents and dark ascospore dots, uni to multi-loculate, globose to subglobose or flask-shaped, with central papilla, ostiole with periphyses. Peridium composed of two layers, outer layer composed of black to reddish brown, thick-walled cells of textura angularis, inner layer composed of hyaline, thin-walled cells of textura angularis. Hamathecium comprising 3–5.5 μm wide, hyaline, hyphae-like, numerous, septate, pseudoparaphyses, constricted at the septa. Asci 100−180 × 28–35 μm (x̄ = 123 × 32 μm, n = 15), 8-spored, bitunicate, fissitunicate, cylindro-clavate or clavate, with a short or long pedicel, apically rounded with an ocular chamber. Ascospores 21–34 × 11.5–15 μm (x̄ = 33 × 14 μm, n = 30), uniseriate at the base, 2–3-seriate at the centre and end, hyaline when immature, gradually pale brown, becoming brown when mature, ellipsoidal, aseptate, straight or slightly curved, widest in the middle, thick-walled, smooth. Asexual morph: Undetermined.
Habitat: Saprobic on dead twigs of Cassia sp.
Known hosts: Citrus sp. (Phillips et al. 2008), Cassia sp. (Hyde et al. 2020).
Known distribution: Cuba (Phillips et al. 2008).
Material examined: Thailand, Chiang Rai Province, Muang District, Tha Sut Subdistrict, on dead twigs of Cassia sp. (Fabaceae), 30 September 2011, M. Doilom (MFLU 19–1560), living culture (MFLUCC 11–0420).
GenBank Accession No: ITS: MN582740, LSU: MN582755, SSU: MN582689.
Notes: Our specimen is similar to the holotype of Barriopsis stevensiana (≡ Barriopsis fusca) which was re-examined by Liu et al. (2012b) and Phillips et al. (2013). Based on phylogenetic analysis of combined LSU, ITS and tef1 sequence data our strain (MFLUCC 11–0420) clusters with the ex-type strain of B. stevensiana (CBS 174.26) with high support (Fig. 100, 100% ML/ 0.98 PP). The holotype was collected on twigs of Citrus in Cuba (Stevens 1926, Phillips et al. 2008, Liu et al. 2012b). This is new geographical record of B. stevensiana on Cassia sp. in Thailand.
Figure Barriopsis stevensiana (MFLU 19–1560, new geographical record). a Ascomata on dead twigs of Cassia sp. b Ascomata cut through horizontally showing the white contents with dark spots. c, d Sections through ascomata. e Peridium. f–k Asci. l–o Ascospores. p Germinated ascospore. Scale bars: c, d = 200 µm, e, g, h, j, k = 20 µm, f, i = 50 µm, l–o = 10 µm, p = 100 µm.
Reference: Hyde KD, de Silva NI, Jeewon R, Bhat DJ, et al. (2020) AJOM new records and collections of fungi: 1–100. Asain Journal of Mycology 3(1):22–294.
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