Botryosphaeriales » Aplosporellaceae » Aplosporella

Aplosporella prunicola

Aplosporella prunicola Damm & Crous, Fungal Diversity 27(1): 39 (2007)

Index Fungorum number: IF504373; Facesofungi number: FoF04955

Saprobic on dead woody twigs of Terminalia chebula. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 320–470 µm high × 500–700 µm diam., (x̅= 380 × 580 µm, n = 5), scattered, immersed, visible as dome-shaped areas on the host surface, multiloculate, coriaceous, globose to subglobose, brown to dark brown with central ostioles. Conidiomata wall 45–90 µm thick, composed of outer layers of dark brown cells and inner layers of hyaline, thick-walled cells of textura angularis. Paraphyses 1.4–2 µm wide, cylindrical to filiform, septate, unbranching. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 5–8 × 2.5–4.4 μm (x̅= 6.3 × 3 μm, n = 10), holoblastic, phialidic, determinate, discrete, oblong to ampulliform, hyaline, smooth-walled, arising from stratum. Conidia 19–23 × 10–12 μm (x̅= 21 × 11 μm, n = 30), straight, initially hyaline, becoming brown to dark brown at maturity, ellipsoid or oval, one-celled, aseptate, ends rounded, thick and smooth-walled.

Culture Characters: Conidia germinating on PDA within 24 h at room temperature (25 °C). Germ tubes produced from the side of conidia. Colonies on PDA, reaching 50 mm diameter after two weeks at 20–25 ℃, mycelia superficial, circular, fluffy, pale gray, reverse grayish with dark brown spots.

Material examined: China, Yunnan Province, Baoshan, on dead woody twigs of Terminalia chebula (Combretaceae), 13 July 2020, G.C. Ren, BS33 (HKAS 122712), living culture KUMCC 21-0518.

Known distribution: On dead branch of Prunus persica var. nucipersica (Rosaceae) from South Africa (Damm et al. 2007), Ficus septica (Moraceae) and Zanthoxylum bungeanum (Rutaceae) in China (Yuan et al. 2020, Li et al. 2023), on dead woody twigs of Terminalia chebula (Combretaceae) in China (This study).

GenBank numbers – LSU: OQ170838, ITS: OQ158918, tef1-α: OR613410.

Notes – In our phylogenetic analysis, our new collection KUMCC 21-0518 clustered with Aplosporella prunicola (CBS 121167, STEU 6326, STEU 6327) and Aplosporella yalgorensis (MUCC 511, MUCC 512) with 96% ML bootstrap support and 1.00 BYPP value. Our isolate shares similar characteristics with A. prunicola in having globose to subglobose, coriaceous conidiomata, holoblastic, hyaline conidiogenous cell and initially hyaline, becoming brown to dark brown, ellipsoid, one-celled, aseptate conidia (Damm et al. 2007, Yuan et al. 2020). A comparison of LSU and ITS sequence data reveals no significant difference between our new isolate and A. prunicola. Therefore, we identify our collection as the first record of A. prunicola on Terminalia chebula (Combretaceae) from Yunnan Province, China.

 

 

Figure 1. Phylogram generated from maximum likelihood analysis based on LSU, ITS and tef1-α sequence data representing family Aplosporellaceae.

  

 

Figure 2. Aplosporella prunicola (HKAS 122712). a Material examined. b Conidiomata on the natural wood surface. c Sections through conidioma. d, e Conidioma wall. f Paraphyses. g–i Conidiogenous cells and developing conidia. j–q Conidia. r Germinated conidium. s, t Culture characters on PDA (s = from above, t = from below). Scale bars: c = 200 μm, d, e = 50 μm, f–q = 10 μm, r = 20 μm, s, t = 30 mm.

 

References

1. Ren GC, Jayasiri SC, Tibpromma S, De Farias ARG, et al. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15(1), 954–1082, Doi 10.5943/mycosphere/15/1/8

 

Recent Genus

Amphisphaeria
Paraeutypella
Halorosellinia

Recent Species

Torula chromolaenae
Periconia delonicis
Neopestalotiopsis theobromicola