Botryosphaeriales » Aplosporellaceae » Aplosporella

Aplosporella javeedii

Aplosporella javeedii Jami, Gryzenh., Slippers & M.J. Wingf., Fungal Biology 118(2): 174 (2013)

Index Fungorum number: IF803637         Facesoffungi number: FoF07383

 

Sexual morph: Undetermined. Asexual morph: Conidiomata 210–290 µm high, 270–680 µm diameter, pycnidial, rounded or valsoid, stromatic, immersed to erumpent through host epidermis, becoming raised, superficial, dark brown to black, subglobose to dome-shaped, discoid or irregular in shape, 1–3-loculate, ostiolate, with a single ostiole throughout stroma. Locules quadrilateral to subglobose, or irregular in shape, 2–3 subdivided chamber separated by invaginations with common walls, the developing invaginations frequently present. Pycnidial walls 25–60 µm wide, thick-walled, composed of two type cells, outer layers comprising several cell layers, of large, broad, thick-walled, dark brown to black cells, arranged in a textura angularis to textura globulosa, inner layers comprising 1–3 layers of small, flattened, light brown pseudoparenchymatous cells paler towards the inner region, arranged in a textura prismatica to textura angularis; interstitial walls of a locule composed of several cell layers of flattened, light brown to brown pseudoparenchymatous cells, arranged in textura prismatica or palisade-like. Paraphyses 28–111 µm long, 1.4–3.2 µm (x̄ = 68.4 × 2.1 µm, n = 40) wide, hyaline, indistinctly septate, thin-walled, filiform, with rounded tip, filamentous, associated with blastic conidiogenesis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells (5.5–)15–28(– 31.5) × (2.5–)3–5.7(–7.7) µm (x̄ = 19.7 × 4.1 µm, n = 30), raising from the inner cavity, holoblastic, monophialidic, hyaline, smooth, short cylindrical to flask-shaped. Conidia 15–27 × 7–12 µm (x̄ = 21.3 × 10 µm, n = 100), initial hyaline, becoming brown to dark brown when mature, ellipsoidal to oblong with rounded ends, or subcylindrical, aseptate, reticulate.

 

Culture characteristics: Colonies on PDA fast growing, reaching 90 mm diameter, after 1 week at 20–25 °C, colonies medium sparse to dense, with aerial mycelia, circular, flat, surface slightly rough with edge entire, margin well defined, cottony to fairly fluffy, with standing tufts; 156 colony from above and below, white to cream at the beginning, becoming black after 4 weeks; not producing pigments on PDA.

 

Habitat: Saprobic on dead hanging twigs of Acer palmatum.

 

Known hosts: Acer buergerianum (Aceraceae), Albizia julibrissin (Fabaceae), Broussonetia papyrifera (Moraceae), Celtis africana (Cannabaceae), Chaenomeles sinensis (Rosaceae), Gleditsia sinensis (Fabaceae), Juglans regia (Juglandaceae), Juniperus chinensis (Cupressaceae), Morus alba (Moraceae), Searsia lancea (Anacardiaceae), Styphnolobium japonicum (Fabaceae), Ziziphus jujuba (Rhamnaceae) (Jami et al. 2013, Fan et al. 2015b, Dou et al. 2017a, Jia et al. 2019).

 

Known distribution: China, Beijing, Gansu, Henan, Heilongjiang, Shanxi, Yunnan (Fan et al. 2015b, Dou et al. 2017a, Jia et al. 2019), South Africa, Gauteng (Jami et al. 2013).

 

Material examined: China, Yunnan Province, Kunming, Panlong, Ciba, on dead hanging twigs of Acer palmatum (Aceraceae), 28 November 2015, R. Phookamsak, COE008, KUN-HKAS 102211, new host and geographical record, living culture, KUMCC 18–0134.

 

GenBank Accession No: ITS: MH974687, TEF1: MT005776.

Notes: An NCBI BLASTn search of ITS sequence data shows that our strain (KUMCC 18–0134) is identical to Aplosporella javeedii with 100% similarity. Phylogenetic analyses of concatenated ITS and TEF1 sequence dataset also indicated that our taxon has a close affinity with A. javeedii with strong support (Fig. 97, 100% ML and 1.00 PP). Furthermore, our collection (KUNHKAS 102211) shares a size range of conidia and conidial characters with the type and other representative specimens (Jami et al. 2013, Fan et al. 2015b). Therefore, we identify our collection as A. javeedii. This species is collected from dead hanging twigs of Acer palmatum in Yunnan, China for the first time.

 

 

Figure  Aplosporella javeedii (KUN-HKAS 102211, new host and geographical record). a, b Appearance of conidiostromata on host substrate. c, d Section through conidiostroma. e, f Section through pycnidial wall. g Paraphyses. h–j Conidiogenous cells embedded in paraphyses. k–q Variation in shape of conidia. r Conidia showing reticulate, rough-walled. Scale bars: a = 1000 µm, b = 500 µm, c, d = 100 µm, e–g = 50 µm, h–r = 10 µm.

Reference: Hyde KD, de Silva NI, Jeewon R, Bhat DJ, et al. (2020) AJOM new records and collections of fungi: 1–100. Asain Journal of Mycology 3:22–294.

 

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