Aplosporella artocarpi
Aplosporella artocarpi Trakun., L. Lombard & Crous, Persoonia 34: 91 (2014)
Index Fungorum number: IF810167; Facesofungi number: FoF13876
Saprobic on dead woody twigs of Hevea brasiliensis. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 270–330 μm high × 320–450 μm diameter (x̅ = 300 × 370 μm, n = 5), scattered, immersed, loculate, coriaceous, globose to subglobose, brown to dark brown with central ostioles. Conidiomatal wall 22–90 μm wide, composed of several layers of thick-walled, dark brown cells of textura angularis. Hamathecium comprising 1.5–2 μm wide, cylindrical to filiform, septate, branching paraphyses. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 3.8–8.3 × 2.2–3.7 μm (x̅ = 5.7 × 3.1 μm, n = 10), enteroblastic, phialidic, determinate, discrete, oblong to ampulliform, hyaline, smooth-walled, arising from stratum. Conidia 15.5–19 × 8.5–10.5 μm (x̅ = 18× 10 μm, n = 30), straight, initially hyaline, becoming brown to dark at maturity, ellipsoid or oval, one-celled, aseptate, rounded ends, thick and smooth-walled.
Culture Characters: Conidia germinating on PDA within 24 h at room temperature. Colonies on PDA, reaching 50 mm diameter after two weeks at 20–25℃, mycelia superficial, circular, velvety, fluffy, dense, granular, white on the surface, reverse white with grey spots.
Material examined: Thailand, Tak Province, on dead woody twigs of Hevea brasiliensis (Euphorbiaceae), 21 April 2019, G.C. Ren, T701 (HKAS 122767), living culture KUMCC 21-0654; ibid., DC07 (HKAS 122768), living culture KUMCC 21-0527.
Known distribution: On twigs of Artocarpus heterophyllus (Moraceae), Chromolaena odorata (Asteraceae), and Hevea brasiliensis (Euphorbiaceae) in Thailand (Trakunyingcharoen et al., 2015, this study).
GenBank numbers: KUMCC 21-0654: LSU: OQ170836, ITS: OQ158916; KUMCC 21-0527: LSU: OQ170837, ITS: OQ158917.
Note: Multigene phylogenetic analysis shows that our strains (KUMCC 21-0654, KUMCC 21-0527) clustered with Aplosporella artocarpi (CPC 22791 and KUMCC 21-0460), A. abexaminans (NFCCI:5010), A. chromolaenae (MFLUCC 17-1517), and Bagnisiella examinans (CBS 551.66), with bootstrap values support lower than 92% ML and 1.00 BYPP. Comparison of LSU and ITS sequence data reveals there is no significant difference between our new isolates (KUMCC 21-0654, KUMCC 21-0527) and Aplosporella artocarpi (CPC 22791 and KUMCC 21-0460), A. abexaminans (NFCCI:5010), A. chromolaenae (MFLUCC 17-1517), and Bagnisiella examinans (CBS 551.66) isolates. A comparison of tef1-α nucleotide shows 0.38% (1/266) differences between our new isolates (KUMCC 21-0527) and Aplosporella artocarpi (CPC 22791). Sequences for tef1-α are lacking for A. abexaminans, A. chromolaenae, and Bagnisiella examinans. Bagnisiella examinans was introduced as a sexual morph and clustered within Aplosporella in this study and previous studies (Wijayawardene, Crous, Kirk, et al., 2014). Morphological characters examined that the isolates of our study largely overlap with Aplosporella artocarpi (Trakunyingcharoen et al., 2015). Therefore, we identify our collection as the first record of Aplosporella artocarpi on Hevea brasiliensis (Euphorbiaceae) from Thailand.
Figure 1. Phylogram generated from maximum likelihood analysis based on LSU, ITS and tef1-α sequence data representing family Aplosporellaceae.
Figure 2. a, b Conidiomata on the natural wood surface. c, d Sections through a conidioma. e Conidioma wall. f Hamathecium. g–i Conidiogenous cells and developing conidia. j–m Conidia. n, o Culture characters on PDA. Scale bars: c = 100 μm, e = 20 μm, f–m = 10 μm, n, o = 30 mm.
References
- Ren GC, Jayasiri SC, Tibpromma S, De Farias ARG, et al. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15(1), 954–1082, Doi 10.5943/mycosphere/15/1/8
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