Conlarium aquaticum

Conlarium aquaticum W. Dong., H. Zhang & K.D. Hyde, Fungal Diversity 85: 75–110 (2017)

Index Fungorum number: IF553759         Facesoffungi number: FoF03337

Sexual morph: Undetermined. Asexual morph: Colonies sporodochial, broadly punctiform, scattered to gregarious, visible as dark brown to black, velvety, shiny colony on the host surface. Mycelium mostly immersed in host substrate, hyaline to light brown, branched, septate, thin- and smooth-walled hyphae. Conidiophores 142–192 × 4.4–5.8 μm (x̄ = 167.1 × 5.1 μm, n = 20), semi-macronematous or macronematous, mononematous, septate, unbranched, straight or flexuous, brown to dark brown. Conidiogenous Cells 15–20 × 3.5–4.9 μm (x̄ = 15.6 × 4.2 μm, n = 20), monoblastic, holoblastic, integrated, determinate, cylindrical, hyaline to pale brown, smooth. Conidia 37–50 × 28–28.5 μm (x̄ = 43.4 × 33.4 μm, n = 20), acrogenous, dictyosporous, muriform, globose to subglobose or irregular in shape, initially hyaline, becoming brown to dark brown when mature, multi-septate, sectored, with small air bubble-like in each cell, slightly constricted at the septa. Conidia secession schizolytic.

Habitat: Saprobic on dead culm of Thysanolaena maxima.

Known hosts: Submerged wood in freshwater (Zhang et al. 2017), Thysanolaena maxima (Hyde et al. 2020).

Known distribution: Thailand (Zhang et al. 2017), China (Hyde et al. 2020).

Material examined: China, Yunnan Province, Xishuangbanna, Mengla County, Xishuangbanna Tropical Botanical Garden (XTBG), on dead stems of Thysanolaena maxima (Poaceae), 22 April 2017, R. Phookamsak, IS005 (MFLU 20–0139, new host record), living culture, KUMCC 18–0189.

GenBank Accession No: ITS: MN994320, LSU: MN994322, SSU: MN994323, TEF1: MT005780 (KUMCC 18–0189A); ITS: MN994328, LSU: MN994330, SSU: MN994331, TEF1: MT005779 (KUMCC 18–0189B).

Notes: Our new collection has a similar morphology with the type species of Conlarium aquaticum in having brown to dark brown, muriform, multi-septate, sectored conidia (Zhang et al. 2017). However, the conidia of the new collection are slightly smaller than the type (MFLU 20–0139: 37–50 × 28–28.5 μm versus 45–70 × 20–57 μm: MFLU 15–2703; Zhang et al. 2017). Phylogenetic analyses of a combined LSU, SSU and ITS sequence dataset indicated that our strains form a strongly supported subclade with C. aquaticum (MFLUCC 15–0992; 98% ML and 1.00 PP). A comparison of the ITS sequence between these strains reveals less than 1.5% nucleotide base differences, which demonstrate that our new collection is conspecific with C. aquaticum (MFLU 15–2703) (Jeewon & Hyde 2016). Therefore, we report our new collection as C. aquaticum which is collected from Thysanolaena maxima in Yunnan, China for the first time in this study (Hyde et al. 2020).