Amphisphaeriales » Sporocadaceae » Pestalotiopsis

Pestalotiopsis pandanicola

Pestalotiopsis pandanicola Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [145] (2018)

Index Fungorum number: IF554519         Facesoffungi number: FoF04529


Sexual morph: Undetermined. Asexual morph: Conidiophores reduced to conidiogenous cells. Conidiogenous cells discrete, holoblastic, simple, filiform, smooth, thin-walled, hyaline, and long. Conidia fusoid to ellipsoid, straight to slightly curved, 4-septate, 13–18 × 2.5–4.5 µm ( = 15.1 × 3.6 µm, n = 30), basal cell conic to obconic with obtuse end, hyaline, thin and verruculose, 2–4 µm long ( = 3.1 µm), with three median cells, doliiform, concolorous, yellow to pale brown, septa, together 8–11 µm long ( = 9.5 µm); second cell from base, pale brown, 2–4 µm long ( = 3 µm); third cell, pale brown, 2.5–4 µm long ( = 3 µm); fourth cell, pale brown, 5–6.5 µm long ( = 3.1 µm), apical cell hyaline, conic to sometimes cylindrical, 2–3 µm long ( = .4 µm), with (2–)3 tubular apical appendages; appendages arising from apex of the apical cell, unbranched, 3.5–8 µm long ( = 6.7 µm); basal appendage present, 1–4 µm long ( = 2.7 µm), single, tubular, centric.


Culture characteristics: Conidia germinating on MEA within 12 h. Colonies on MEA reaching 9 cm diam., after 7 days at room temperature, circular, edge entire, whitish to pale yellow, with dense, aerial mycelium on surface, fruiting bodies black; reverse of culture whitish to pale  yellow.


Habitat: Saprobic on dead leaves of Pandanus sp.


Known distribution: Thailand


Material examined: THAILAND, Krabi Province, Mueang Krabi District, on Pandanus sp., 14 December 2015, S. Tibpromma KB030 (MFLU 16–1908, holotype; HKAS 96257, isotype); ex-type living culture, MFLUCC 16–0255 = KUMCC 16–0140.


GenBank numbers: LSU: MH376735, ITS: MH388361, TEF1: MH388396, ACT: MH412716, TUB2: MH412723.


Notes: Pestalotiopsis pandanicola clusters with P. krabiensis in the phylogenetic analysis, which has been described from the same location. Therefore, we propose P. pandanicola as a new species in Pestalotiopsis based on morphology and phylogenic analysis which discussion at above. In a BLASTn search on NCBI GenBank, the closest matches of TEF1 sequence of MFLUCC 16–0255 is P. adusta strain with 94% identity to the strain MFLUCC 10–0146 (JX399071), while the closest matches with the TUB2 sequence were with 99% P. uvicola strain HHL-AG (HM573238).