Pestalotiopsis pandanicola
Pestalotiopsis pandanicola Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [145] (2018)
Index Fungorum number: IF554519 Facesoffungi number: FoF04529
Sexual morph: Undetermined. Asexual morph: Conidiophores reduced to conidiogenous cells. Conidiogenous cells discrete, holoblastic, simple, filiform, smooth, thin-walled, hyaline, and long. Conidia fusoid to ellipsoid, straight to slightly curved, 4-septate, 13–18 × 2.5–4.5 µm (x̅ = 15.1 × 3.6 µm, n = 30), basal cell conic to obconic with obtuse end, hyaline, thin and verruculose, 2–4 µm long (x̅ = 3.1 µm), with three median cells, doliiform, concolorous, yellow to pale brown, septa, together 8–11 µm long (x̅ = 9.5 µm); second cell from base, pale brown, 2–4 µm long (x̅ = 3 µm); third cell, pale brown, 2.5–4 µm long (x̅ = 3 µm); fourth cell, pale brown, 5–6.5 µm long (x̅ = 3.1 µm), apical cell hyaline, conic to sometimes cylindrical, 2–3 µm long (x̅ = .4 µm), with (2–)3 tubular apical appendages; appendages arising from apex of the apical cell, unbranched, 3.5–8 µm long (x̅ = 6.7 µm); basal appendage present, 1–4 µm long (x̅ = 2.7 µm), single, tubular, centric.
Culture characteristics: Conidia germinating on MEA within 12 h. Colonies on MEA reaching 9 cm diam., after 7 days at room temperature, circular, edge entire, whitish to pale yellow, with dense, aerial mycelium on surface, fruiting bodies black; reverse of culture whitish to pale yellow.
Habitat: Saprobic on dead leaves of Pandanus sp.
Known distribution: Thailand
Material examined: THAILAND, Krabi Province, Mueang Krabi District, on Pandanus sp., 14 December 2015, S. Tibpromma KB030 (MFLU 16–1908, holotype; HKAS 96257, isotype); ex-type living culture, MFLUCC 16–0255 = KUMCC 16–0140.
GenBank numbers: LSU: MH376735, ITS: MH388361, TEF1: MH388396, ACT: MH412716, TUB2: MH412723.
Notes: Pestalotiopsis pandanicola clusters with P. krabiensis in the phylogenetic analysis, which has been described from the same location. Therefore, we propose P. pandanicola as a new species in Pestalotiopsis based on morphology and phylogenic analysis which discussion at above. In a BLASTn search on NCBI GenBank, the closest matches of TEF1 sequence of MFLUCC 16–0255 is P. adusta strain with 94% identity to the strain MFLUCC 10–0146 (JX399071), while the closest matches with the TUB2 sequence were with 99% P. uvicola strain HHL-AG (HM573238).
Figure X. Pestalotiopsis pandanicola (MFLU 16-1908, holotype). a Colonies on dead leaves of Pandanus sp. b, c Conidiogenous cells with conidiophores and conidia. d–h Conidia. i Germinating conidium. j, k Colony on MEA from above and below. l Conidiogenous cells with conidia formed in culture. m Conidia formed in culture. Scale bars: b–d = 10 µm, e–i, l, m = 5 µm.
Reference: Tibpromma S, Hyde KD, McKenzie EHC, et al. (2018) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity 93: 1–160.
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