Amphisphaeriales » Sporocadaceae » Pestalotiopsis

Pestalotiopsis krabiensis

Pestalotiopsis krabiensis Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [143] (2018)

Index Fungorum number: IF554518         Facesoffungi number: FoF04528

 

Sexual morph: Undetermined. Asexual morph: Conidiophores short, unbranched, reduced to conidiogenous cells. Conidiogenous cells discrete, holoblastic, simple, filiform, smooth, thin-walled, hyaline. Conidia fusoid to ellipsoid, straight to slightly curved, 4-septate, 19–25 × 4–6 µm ( = 21 × 5 µm, n = 30), basal cell conic to obconic with obtuse end, hyaline, thin-walled, verruculose, 3.5–5 µm long ( = 4 µm); three median cells, doliiform, concolorous, hyaline becoming yellow–white with age, septa and periclinal walls darker than rest of the cell, together 13–15 µm long ( = 13.7 µm); second cell from base 3–5 µm long ( = 4 µm); third cell 4–5.5 µm long ( = 4.6 µm); fourth cell 4–5 lm long ( = 4.3 µm); apical cell hyaline, conic 4–5.5 µm long ( = 4.5 µm), with 2(–3) tubular apical appendages; appendages arising from the apex of the apical cell, unbranched, 11–19 µm long ( = 15 µm); single basal appendage usually present, 2–4 µm long ( = 2.6 µm), tubular, unbranched, centric.

 

Culture characteristics: Conidia germinating on MEA within 12 h. Colonies on MEA reaching 9 cm diam., after 7 days at room temperature, edge undulate, whitish, aerial mycelium on surface, curled, fruiting bodies black after 2 months, concentric, floosy, velvety; reverse of culture  yellow–white.

 

Habitat: Saprobic on dead leaves of Pandanus sp.

 

Known distribution: Thailand.

 

Material examined: THAILAND, Krabi Province, Mueang Krabi District, on Pandanus sp., 14 December 2015, S. Tibpromma KB041 (MFLU 16–1919, holotype; HKAS 96268, isotype); ex-type living culture, MFLUCC 16–0260 = KUMCC 16–0141.

 

GenBank numbers: LSU: MH376734, ITS: MH388360, TEF1: MH388395, ACT: MH412715, TUB2: MH412722.

 

Notes: In the phylogenetic analysis, Pestalotiopsis krabiensis forms a sister group to P. pandanicola. Pestalotiopsis krabiensis has larger conidia (21.1 × 5.2 µm) with mostly 2 apical appendages, while P. pandanicola has smaller conidia (15.1 × 3.6 µm) with 3 apical appendages. In a comparison of the 517 TEF1 nucleotides of P. pandanicola and P. krabiensis differed 1 bp (0.19%), 475 TUB2 nucleotides differed 17 bp (3.57%) and 577 ITS (5.8S) nucleotides differed 1 bp (0.17%) which justifies these two isolates as two distinct taxa. In a BLASTn search on NCBI GenBank, the closest matches of TEF1 sequence of MFLUCC 16-0260 is P. adusta strain with 99% identity to the strain MFLUCC 10–0146 (JX399071), while the closest matches with the TUB2 sequence were with 99% P. adusta strain LPJZ02 (KJ885549).