Amphisphaeriales » Sporocadaceae » Neopestalotiopsis

Neopestalotiopsis phangngaensis

Neopestalotiopsis phangngaensis Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [142] (2018)

Facesoffungi number: FoF04527

 

Saprobic on dead leaves of Pandanus sp. Sexual morph: Undetermined. Asexual morph: Conidiophores reduced to conidiogenous cells. Conidiogenous cells enteroblastic, annellidic, filiform, ampulliform, smooth, thin-walled, hyaline, short. Conidia fusoid to ellipsoid, straight to slightly curved, 4-septate, 18–25 × 6–7.5 μm ( x̄ = 21 × 6.6 μm, n = 20); basal cell conic to obconic with obtuse end, hyaline, verruculose, 3–5 μm long (x̄ = 4.3 μm); three median cells doliiform, concolorous, hyaline to subhyaline, becoming brown with age, septa and periclinal walls darker than rest of cells, together 13–15 μm long (x̄ = 14 μm); second cell from base 3.5–5 μm long (x̄ = 4.6 μm); third cell 2.5–5 μm long (x̄ = 4.5 μm); fourth cell 4–5 μm long (x̄ = 4.5 μm); apical cell hyaline, conic 3–4.5 μm long (x̄ = 3.5 μm), with 3 tubular apical appendages; appendages unbranched, 16–24.5 μm long (x̄ = 18 μm); basal appendage present 3–6 μm long (x̄ = 3.9 μm), rarely absent, tubular, unbranched, centric.

 

Culture characteristics: Conidia germinating on MEA within 12 hr. Colonies on MEA reaching 9 cm diam., after 7 days at room temperature, edge undulate, whitish, aerial mycelium on surface, floosy, velvety; reverse of culture yellow white.

 

Material examined: THAILAND, Phang Nga Province, Thap Put District, on Pandanus sp., 20 December 2017, N. Chaiwan P18 (MFLU 18-0037, holotype; HKAS 101796, isotype); ex-type living culture, MFLUCC 18-0119, GenBank numbers LSU: MH376728; ITS: MH388354; SSU: MH388322; TEF1: MH388390; TUB2: MH412721.

 

Notes: Neopestalotiopsis phangngaensis is well-separated from other species of Neopestalotiopsis in the combined gene phylogenetic analyses (Fig. 105). Neopestalotiopsis phangngaensis is similar to N. eucalypticola isolated from Eucalyptus globules in country name. However, N. eucalypticola has 1–2, long tubular apical appendages, which are sometimes branched (Maharachchikumbura et al. 2016), while N. phangngaensis has 5 tubular apical appendages which are unbranched. In a comparison of the 512 TEF1 nucleotides of N. eucalypticola and N. phangngaensis differed 5 bp (0.97%) and 441 TUB2 nucleotides differed 26 bp (5.89%). In a blastN search on NCBI GenBank, the closest matches of TEF1 sequence of KUMCC 17-0175 is Neopestalotiopsis sp. with 90% identity to the strain MMf-0011 (LC184190), while the closest matches with the TUB2 sequence were with 99% Pestalotiopsis sp. strain HGUP4077 (KF179298).

 

Figure X. Neopestalotiopsis phangngaensis (MFLU 18-0037, holotype). a Colonies on dead leaves of Pandanus sp. b, c Conidiogenous cells with conidiophores and conidia. d-f Conidia. g Germinating conidium. h, i Colony on MEA from above and below. Scale bars: b-g = 5 μm.

 

Reference: Tibpromma S, Hyde KD, McKenzie EHC, Bhat DJ, et al. (2018) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity 92:1–160.

 

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