Amphisphaeriales » Sporocadaceae » Neopestalotiopsis

Neopestalotiopsis pandanicola

Neopestalotiopsis pandanicola Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [141] (2018)

Index Fungorum number: IF554516         Facesoffungi number: FoF04526

 

Sexual morph: Undetermined. Asexual morph: Conidiomata 280–450 µm, acervulus, subepidermal in origin, with basal stroma, thin-walled comprising hyaline to pale brown cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells oval, smooth, thin-walled, hyaline, short. Conidia fusoid to ellipsoid, straight to slightly curved, 4-septate, 27–35 × 7.5–11 µm (x̅ = 31 × 9 µm, n = 20); basal cell conic to obconic with obtuse end, sub-hyaline to pale brown, thin-walled and verruculose, 3.5–8 µm long (x̅ = 6 µm); three median cells, doliiform, concolorous, brown, periclinal walls darker than rest of the  cell, together 17–25 µm long (x̅ = 21 µm); second cell  from base 4.5–12.5 µm long (x̅ = 8 µm); third cell 5–8 µm  long (x̅ = 7 µm); fourth cell 6–10 µm long (x̅ = 8 µm); apical cell hyaline, conic to cylindrical 3–6 µm long (x̅ = 4 µm), with 2(–3) tubular apical appendages; unbranched, 9.5–26 µm long (x̅ = 17 µm); with single basal appendage 3–6 µm long (x̅ = 5 µm), rarely absent, tubular, unbranched, centric.

 

Culture characteristics: Conidia germinating on PDA within 12 h. Colonies on PDA reaching 9 cm diam., after 7 days at room temperature, edge undulate, whitish, aerial mycelium on surface, floosy, velvety; reverse of culture yellow–white.

 

Habitat: Saprobic on fallen dead and decaying leaves of Pandanus sp.

 

Known distribution: China

 

Material examined: CHINA, Yunnan Province, Xishuangbanna, on fallen dead and decaying leaves of Pandanus sp., 12 November 2016, T. Aluthwaththa XTBG09 (HKAS 99617, holotype); ex-type living culture, KUMCC 17–0175 = MFLUCC 17–2261.

 

GenBank numbers: LSU: MH376727, SSU: MH388321, TEF1: MH388389, TUB2: MH412720.

 

Notes: Neopestalotiopsis pandanicola is morphologically similar to N. javaensis Maharachch., K.D. Hyde & Crous. Conidiogenous cells of N. pandanicola are filiform and short, while the conidiogenous cells of N. javaensis are ampulliform to lageniform and rugose-walled. Neopestalotiopsis pandanicola is phylogenetically closely related to N. chiangmaiensis. However, this species differs from N. pandanicola by has smaller conidia (20 × 9 µm) and apical appendages. In a comparison of the 490 TEF1 nucleotides of N. pandanicola and N. chiangmaiensis differed 23 bp (4.69%). In a BLASTn search on NCBI GenBank, the closest matches of TEF1 sequence of KUMCC 17–0175 is N. clavispora with 99% identity to the strain TOR-802-803-804 (KU096881), while the closest matches with the TUB2 sequence were with 99% identical N. clavispora strain NM16311a (LC209221).

 

Figure X. Neopestalotiopsis pandanicola (HKAS 99617, holotype). a Appearance of conidiomata on dead leaves of Pandanus sp. b Section of conidiomata. c Conidiogenous cells with conidiophores and conidia. d–g Conidia. h Germinating conidium. i, j Colonies on MEA from above and below. Scale bars: b = 100 µm, c–h = 5 µm.

 

Reference: Tibpromma S, Hyde KD, McKenzie EHC, et al. (2018) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity 93: 1–160.

 

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