Amphisphaeriales » Sporocadaceae » Neopestalotiopsis

Neopestalotiopsis chiangmaiensis

Neopestalotiopsis chiangmaiensis Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [139] (2018)

Index Fungorum number: IF554515            Facesoffungi number: FoF04525

 

Saprobic on dead leaves of Pandanus sp. Sexual morph: Undetermined. Asexual morph: Conidiomata sporodochia on surface of substrate, scattered, dark brown, dull. Conidiophores reduced to conidiogenous cells. Conidiogenous cells holoblastic, filiform, smooth, thin-walled, hyaline, short. Conidia fusoid to ellipsoid, straight to slightly curved, 4-septate, 18–22 × 8–11 μm ( x̄ = 20 × 9 μm, n = 20); basal cell conic to obconic with obtuse end, subhyaline, thin-walled and verruculose at the basal cell, 4–5 μm long (x̄ = 4.2 μm); three median cells, doliiform, concolorous, yellow to brown, together 12–17 μm long (x̄ = 14 μm); second cell from base, yellow, 3.5–5 μm long (x̄  = 4 μm); third cell, yellow brown, 3.5–6 μm long (x̄  = 4.6 μm); fourth cell, brown, 3–5 μm long (x̄  = 4.4 μm); apical cell hyaline to subhyaline, conic, 3–5 μm long (x̄ = 4 μm), with (2–)3 tubular apical appendages; appendages unbranched, 4–28 μm long (x̄  = 14 μm); basal appendage present, 3–5 μm long (x̄  = 4 μm).

 

Culture characteristics: Conidia germinating on MEA within 12 hr. Colonies on MEA reaching 9 cm diam., after 7 days at room temperature, edge undulate, whitish, aerial mycelium on surface, floosy, velvety; reverse of culture yellow.

 

Material examined: THAILAND, Chiang Mai Province, Mae Taeng District, Mushroom Research Foundation, on Pandanus sp., 16 December 2017, S. Tibpromma P07 (MFLU 18-0026, holotype; HKAS 101795, isotype); ex-type living culture, MFLUCC 18-0113, GenBank numbers TEF1: MH388404; TUB2: MH412725.

 

Notes: Based on multi-gene phylogenetic analysis, Neopestalotiopsis chiangmaiensis clusters with N. pandanicola and N. cubana Maharachch., K.D. Hyde & Crous (Fig. 105). Neopestalotiopsis pandanicola has 2(–3) tubular apical appendages 9.5–26 μm long, while N. cubana has 2–4 tubular apical appendages (19–)21–27(–28) μm long (Maharachchikumbura et al. 2014). Neopestalotiopsis chiangmaiensis has (2–)3 tubular apical appendages; appendages unbranched, 4–28 μm long. In a comparison of the 512 TEF1 nucleotides of N. chiangmaiensis and N. cubana differed 6 bp (1.17%) and 441 TUB2 nucleotides differed 3 bp (0.68%). In a blastN search on NCBI GenBank, the closest matches of TEF1 sequence of MFLUCC 18-0113 is N. clavispora with 98% identity to the strain TOR-802-803-804 (KU096881), while the closest matches with the TUB2 sequence were with 99% identical N. clavispora strain JT11 (MG740724).

 

Figure X. Neopestalotiopsis chiangmaiensis (MFLU 18-0026, holotype). a Appearance of conidiomata on dead leaves of Pandanus sp. b Conidiogenous cells with conidiophores and conidia. c-h Conidia. i Germinating conidium. j, k Colony on MEA from above and below. Scale bars: b-i = 5 μm.

 

Reference: Tibpromma S, Hyde KD, McKenzie EHC, Bhat DJ, et al. (2018) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity 92:1–160.

 

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