Amphisphaeriales » Pestalotiopsidaceae » Ciliochorella

Ciliochorella sp.

Ciliochorella sp. Gomdola, K.D. Hyde & Jayaward.,

Index Fungorum number , Facesoffungi number: FoF                             

Saprobic on the leaves of Jasminum sp. Sexual morph: Not observed. Asexual morph: Coelomycetous. Conidiomata in cross-section 1000–1100 μm diam., 370–380 μm high ( = 1042 × 373 μm, n = 5), acervulus, semi-immersed, carbonaceous, solitary, uniloculate, black. Conidiomata wall 40–53 μm diam. (= 46.7 μm, n = 10), consisting of several layers of pseudoparenchymatous cells of textura angularis, outer layers dark brown, inner layers pale brown to hyaline. Conidiophores indistinct, often reduced to conidiogenous cells. Conidiogenous cells phialidic, (5.2–)6.7–8.5(–9.5) × 1.9–2.8 μm (= 7.4 × 2.4 μm, n = 10), formed from the inner-most layer of the wall, hyaline to pale brown, ampulliform, smooth-walled, proliferating enteroblastically. Conidia 11–15 × 2.4–3.8 μm (= 12.9 × 3.3 μm, n = 50) (excluding basal cell), hyaline to pale brown, guttulate, 1-euseptate, smooth-walled, allantoid to sub-cylindrical, or sub-falcate to reniform, apex sometimes broadly obtuse, tapering towards a slightly curved base with a hyaline obconic basal cell 2.8–4.5 μm long (= 3.6 μm, n = 30); conidia bearing 2 apical and 1 basal appendage. Appendages tubular, filiform, flexuous, apical appendages (6.5–)12.5–18.5 μm long (= 16.5 μm, n = 50), basal appendage (2.5–)4–6.5(–8) μm long (= 5.4 μm, n = 50). Appressorium 20 × 18.5 μm, single-celled, cordate to irregular-shaped, hyaline.

Culture characteristics: Colonies on MEA reaching approximately 20 mm diam. after 14 days of incubation at 25 °C, elevation flat, forming concentric rings with an entire margin, mycelium white.

Material examined: Thailand, Chiang Mai Province, Doi Lo district, on fallen dead leaves of Jasminum sp. (Oleaceae), 15 October 2019, D. Gomdola, DG314 (MFLU 23-0388), living culture MFLUCC 23-0239.

GenBank accession numbers: ITS = OR610581, 28S = OR610582.

Notes: Ciliochorella sp. (MFLUCC 23-0239) groups with other Ciliochorella species and forms a separate lineage with the larger subclade in which reside C. dipterocarpi, C. mangiferae, and C. phanericola (97% ML-IQ, 96% MP, 0.88 PP) (Figure 2). The conidial features match the morphological species concept of Ciliochorella. We compared the morphology of Ciliochorella sp. (MFLUCC 23-0239) with that of its phylogenetically closely related, C. phanericola. The conidial shape, color, and size of Ciliochorella sp. (MFLUCC 23-0239) and C. phanericola are mostly similar (Table 1). However, the conidia of Ciliochorella sp. (MFLUCC 23-0239) are 1-euseptate, while those of C. phanericola are 2-septate. Both the apical and basal appendages of Ciliochorella sp. (MFLUCC 23-0239) are shorter than those of C. phanericola (Table 1). The growth rate of Ciliochorella sp. (MFLUCC 23-0239) (2 cm after 14 days) is slower than that of C. phanericola (2.5 cm after 7 days), both grown on MEA and incubated at 25 °C (Hyde et al. 2026). In addition, appressoria were not observed in C. phanericola (Hyde et al. 2016).

Excluding gaps in our aligned untrimmed dataset, in comparison of the inter-species genetic distance of Ciliochorella sp. (MFLUCC 23-0239) and C. phanericola, a difference of 0.34% was seen across ITS (533 nucleotides), but no difference was observed across 28S (868 nucleotides). We were unable to compare the differences across β-tub as Ciliochorella sp. (MFLUCC 23-0239) lacks sequence data for the gene region. Despite several trials using different amplification conditions, we were unable to obtain sequence data for β-tub. Therefore, coupled with morphological description and multi-locus phylogenetic analyses, a PHI test was also conducted to support the taxonomic placement of our isolate (MFLUCC 23-0239). The PHI test of the combined ITS and 28S yielded a threshold exceeding 0.05 (Фw = 1.0), suggesting that no recombination event has occurred.

Nevertheless, despite the PHI test result, we suggest establishing our isolate as Ciliochorella sp. instead of identifying it as a new species due to the lack of sequence data. Further studies focusing on the collection of more Ciliochorella taxa and providing sequence data for protein-coding gene regions (β-tub, Rpb2, tef-1α) will yield better resolution in the phylogenetic trees and contribute to proper species identification.