Austropleospora archidendri

Austropleospora archidendri (Verkley, Göker, and Stielow) Ariyaw. and K.D. Hyde, Fungal Diversity 75: 64 (2015)

Index Fungorum number: IF 551419; Facesoffungi number: FoF 00936.

Saprobic on dead woody twigs of Euphoria longana. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 165–225 µm high × 115–165 µm diam. (x = 190 × 140 µm, n = 10), scattered, immersed, unilocular, coriaceous, globose-to-sub-globose, brown-to-dark-brown with a central ostiole. Ostiole 60–75 × 40–50 µm (x̅ = 70 × 45 µm, n = 5), short papillate, black. Conidiomatal wall 15–25 µm thick, 3–4-layered, composed of brown outer and hyaline inner layers, thin-walled cells of textura angularis. Conidiophores reduced into conidiogenous cells. Conidiogenous cells 3.7–6 × 2.8–3.8 µm (x̅ = 4.2 × 3.4 µm, n = 10), enteroblastic, phialidic, determinate, discrete, doliiform-to-ampulliform, hyaline, smoothwalled, arising from stratum. Conidia 4.8–5.8 × 3–3.6 µm (x̅ = 5.4 × 3.4 µm, n = 30), straight, initially hyaline, guttulate, becoming brown at maturity, sub-globose to ovate, one-celled, rounded ends, thick-walled.

 Culture characteristics: The colonies reached 70–80 mm diam. on PDA at 14 days at room temperature (25–30 ◦C), superficial, flat, circular, medium-dense, rough, fluffy, zonate, raised between margin and center, gray at the margin, white at the center; reverse, zonate, pale gray at the margin, dark gray at the center and zonate.

 Material examined: Thailand, Chiang Mai Province, Yang Piang Omkoi, on dead woody twigs of Euphoria longana, 25 August 2019, G.C. Ren, YP03 (MFLU 22-0042), living culture KUMCC 21-0680. Known distribution: on leaf spot in Archidendron bigeminum (Myanmar), decaying pod of Leucaena sp. (Thailand).

Notes: Austropleospora archidendri was introduced by Ariyawansa et al. (2015). as a new combination of Paraconiothyrium archidendri based on the combined phylogeny of LSU, SSU, β-tubulin, and ITS sequence data. In the present study, the multi-gene phylogenetic analyses indicated that our new strain, KUMCC 21-0680, formed a sister clade with A. archidendri (MFLUCC 17-2429) with 100% ML bootstrap and 0.95 BYPP support . Since the type species lacks SSU and tef 1-α sequences, the nucleotide base pair comparisons of LSU and ITS demonstrated that our new strains are identical to the type of the Austropleospora archidendri strain and other species. Our strain, KUMCC 21-0680, is similar to A. archidendri (CBS 168.77, MFLUCC 17-2429) in having doliiform conidiogenous cells and sub-globose-to-ovate, brown, aseptate conidia. Austropleospora archidendri was reported as a pathogen on A. bigeminum leaves in Thailand and a saprobe on the pods of a Leucaena sp. in Myanmar. Therefore, we report our strain KUMCC 21-0680 as a new record of A. archidendri on woody litter of Euphoria longana in Thailand.

Figure 1. Phylogram generated from ML analysis based on the combined SSU, LSU, ITS, and tef 1- dataset. Bootstrap support values for ML equal to or higher than 75%, and BYPP equal to or greater than 0.90 are shown above the nodes.